POP2 Antibody

Code CSB-PA562843XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) POP2 Polyclonal antibody
Uniprot No.
Target Names
POP2
Alternative Names
POP2 antibody; GABA-T antibody; GABAT1 antibody; HER1 antibody; At3g22200 antibody; MKA23.11Gamma-aminobutyrate transaminase POP2 antibody; mitochondrial antibody; AtGABA-T antibody; EC 2.6.1.96 antibody; Gamma-aminobutyric acid aminotransferase 1 antibody; Protein HEXENAL RESPONSE 1 antibody; Protein POLLEN-PISTIL INCOMPATIBILITY 2 antibody; AtPOP2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana POP2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transaminase that degrades gamma-amino butyric acid (GABA) and uses pyruvate or glyoxylate as amino-group acceptor, but not 2-oxoglutarate. The pyruvate-dependent activity is reversible while the glyoxylate-dependent activity is irreversible. Cannot use beta-alanine, ornithine, acetylornithine, serine, glycine, asparagine, glutamine, glutamate, valine, leucine, isoleucine, methionine, phenylalanine, histidine, lysine, arginine, aspartate, threonine, tyrosine, tryptophan, proline, or cysteine as amino donors. Modulates steady-state GABA levels in diploid pistil cells and the haploid pollen tube. Involved in the formation of a gradient of GABA along the pollen tube path. Involved in the maintenance of the shoot apical meristem (SAM) structure and subsequent adaxial-abaxial axis-dependent development of cotyledons and leaves.
Gene References into Functions
  1. strong connection between GABA metabolism and glyoxylate cycle during stress PMID: 23518583
  2. AtGABA-T is functionally interchangeable with ScGABA-TKG for GABA growth, thermotolerance and limiting production of ROS, regardless of location in mitochondria or cytosol of yeast cells, but AtGABA-T is about half as efficient in doing so as ScGABA-TKG. PMID: 23740823
  3. GABA-T deficiency during salt stress causes root and hypocotyl developmental defects and alterations of cell wall composition. PMID: 23148892
  4. The GABA transaminase deficient mutant pop2-1 is ovsersensitive to NaCl. PMID: 20122158
  5. isolation and characterization of the her1 mutant (encoding GABA-TP) altered in a specific response to E-2-hexenal; study shows that, as a consequence of the mutation in GABA-TP, her1 plants accumulate high GABA levels PMID: 17971036
  6. Data suggest that POP2 mutants suppress the phenotype of succinic semialdehyde dehydrogenase (ssadh) mutants in Arabidopsis. PMID: 18846220
  7. GABA-T possesses both pyruvate- and glyoxylate-dependent activities. PMID: 19264755

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Subcellular Location
Mitochondrion.
Protein Families
Class-III pyridoxal-phosphate-dependent aminotransferase family
Tissue Specificity
Expressed in flowers, siliques, stems, leaves, shoots and roots. Detected in reproductive tissues, in the stigma, style, abscission zone of siliques, stamens and pollen. Not found in pollen tubes or the transmitting tract. In vegetative tissues, found in
Database Links

KEGG: ath:AT3G22200

STRING: 3702.AT3G22200.2

UniGene: At.22661

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