Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ASC1 Polyclonal antibody

Uniprot No.

P38011

Target Names

ASC1

Alternative Names

Guanine nucleotide-binding protein subunit beta-like protein (Receptor for activated C kinase) (Receptor of activated protein kinase C 1) (RACK1) (Small ribosomal subunit protein RACK1) ASC1 CPC2 YMR116C YM9718.15C

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ASC1 protein (2-319aa)

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Located at the head of the 40S ribosomal subunit in the vicinity of the mRNA exit channel, RACK1 serves as a scaffold protein that can recruit other proteins to the ribosome. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Involved in the negative regulation of translation of a specific subset of proteins.

Gene References into Functions

Data provide evidence that Asc1 seems to connect signal transduction not only with the translational machinery, but also with fundamental nuclear processes. PMID: 28982715
These indicate that ribosome-associated Asc1/RACK1 facilitates endonucleolytic cleavage of nonstop mRNA by stalled ribosomes and represses the levels of aberrant products even in the absence of Dom34. We propose that Asc1/RACK1 acts as a fail-safe in quality control for nonstop mRNA. PMID: 27312062
identify Asc1p (aka RACK1) and Rps3p, both stable 40S subunit components, as additional 18S nonfunctional 18S rRNA decay factors PMID: 28956756
It is the paper where ASC1 gene is identified and cloned and named. The effect of ASC1 deletion is also studied. PMID: 9504906
Translation fidelity depends upon Asc1-mediated quality control. PMID: 25792604
These results indicate the intertwined structure of a relatively small and stable protein core and the remaining regions of different levels of flexibility. PMID: 24591271
Genetic interactions between dys1-1 mutants and mutant PKC1 and ASC1, implicate a role for HYP2 (eIF5A homolog) and Asc1 in the maintenance of cell integrity at the translational level. PMID: 23573236
Evidence of the translational regulation of such transcription factors suggests that ribosomal Asc1p is involved in signal transduction pathways and controls the biosynthesis of the respective final transcriptional regulators. PMID: 23071099
The structure reveals an atypical mode of dimerization where monomers intertwine on blade 4, thus exposing a novel surface of the protein to potential interacting partners. PMID: 21704636
Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast PMID: 17591772
RACK1 functions from the ribosome, implying a physical link between the eukaryotic ribosome and cell signaling pathways in vivo. PMID: 19114558

Hide All

Subcellular Location

Cytoplasm.

Protein Families

WD repeat G protein beta family, Ribosomal protein RACK1 subfamily

Database Links

KEGG: sce:YMR116C

STRING: 4932.YMR116C

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Code	CSB-PA339901XA01SVG
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: ASC1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
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ASC1 Antibody

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