GAMMACA2 Antibody

Code CSB-PA928546XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GAMMACA2 Polyclonal antibody
Uniprot No.
Target Names
GAMMACA2
Alternative Names
GAMMACA2 antibody; APFI antibody; At1g47260 antibody; F8G22.2 antibody; Gamma carbonic anhydrase 2 antibody; mitochondrial antibody; AtCA2 antibody; GAMMA CA2 antibody; EC 4.2.1.- antibody; Transcription factor APFI antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GAMMACA2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Enzyme involved in the catabolism of H(2)CO(3) but that does not mediates the reversible hydration of carbon dioxide. Mediates complex I assembly in mitochondria and respiration. Binds HCO(3)-. Required for male fertility during anther development and dehiscence to regulate the secondary thickenings of the endothecial cell wall, probably by modulating H(2)O(2)-dependent lignin polymerization.
Gene References into Functions
  1. At1g47260 is required for complex I assembly and that reproductive development is dependent on the presence of complex I. PMID: 26889912
  2. AT1G47260 contributes to sustaining efficient photosynthesis under ambient (photorespiratory) conditions. PMID: 26148112
  3. At1g47260 is important for complex I assembly in plant mitochondria and respiration. PMID: 15935378
  4. Resuslts suggest that reduction in ROS inhibits H(2)O(2) dependent lignin polymerization in CA2 over-expressing plants, thereby causing male sterility. PMID: 19326245
  5. Plant gammaCAs do not act as carbonic anhydrases but with a related activity possibly contributing to recycle CO(2) in the context of photorespiration. PMID: 19808034

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Subcellular Location
Mitochondrion membrane; Peripheral membrane protein; Matrix side.
Protein Families
Gamma-class carbonic anhydrase family
Tissue Specificity
Constitutively expressed in roots and leaves, with higher levels in flowers, particularly in tapetal tissue of anthers, inflorescence (IM) and floral meristems (FM).
Database Links

KEGG: ath:AT1G47260

STRING: 3702.AT1G47260.1

UniGene: At.397

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