Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GCN2 Polyclonal antibody

Uniprot No.

Q9LX30

Target Names

GCN2

Alternative Names

GCN2 antibody; At3g59410 antibody; F25L23_270 antibody; eIF-2-alpha kinase GCN2 antibody; serine/threonine-protein kinase GCN2 antibody; EC 2.7.11.1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GCN2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 eIF-2-alpha in response to low amino acid availability. Plays a role as an activator of the general amino acid control pathway required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha either to a competitive inhibitor of translation initiation, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, and hence allowing reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs.

Gene References into Functions

In concert with GCN2, TOR allows photo-autotrophic eukaryotes to coordinate the fluxes of carbon, nitrogen, and sulfur for efficient cysteine biosynthesis under varying external nutrient supply PMID: 29079776
Although both ILA and GCN2 genes are necessary to mediate eIF2alpha phosphorylation upon treatments with the aromatic amino acid biosynthesis inhibitor glyphosate, their mutants develop distinct root and chloroplast phenotypes. PMID: 29680783
These results points to an unknown GCN2-dependent factor involved in the cascade of events triggered by glyphosate in plants. PMID: 25603772
AtGCN2 plays pivotal roles in various cellular processes essential for normal growth and development. PMID: 25912940
Analysis of wild-type and GT8359 (null mutant for AtGCN2) plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2alpha was not phosphorylated. PMID: 18603615
GCN2 is activated following wounding and exposure to hormones. GCN2 can phosphorylate eIF2alpha and is essential in amino acid deprivation conditions. PMID: 19108716

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Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily

Tissue Specificity

Expressed in roots, leaves, stems, buds, flowers, siliques and seedlings.

Database Links

KEGG: ath:AT3G59410

STRING: 3702.AT3G59410.2

UniGene: At.34633

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GCN2 Antibody

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