GCN2 Antibody

1. findings demonstrate that TORC1 is an effector of Gcn2 in amino acid signaling, hence defining a novel mechanism by which TORC1 senses amino acid starvation PMID: 28057755
2. GCN2 is required for nitrogen catabolite repression-sensitive nuclear Gln3 localization. Gcn2 functions upstream of Ure2. PMID: 28007891
3. Mutations leading to amino acid substitutions on this surface were identified that activate Gcn2 at low levels of tRNA binding (Gcd- phenotype), while other substitutions block kinase activation (Gcn- phenotype PMID: 25695491
4. To mediate the activation of protein kinase Gcn2, Gcn1 interacts with Rps10. PMID: 25437641
5. Gcd- substitutions enhance YKD-KD interactions in vitro, whereas Gcn- substitutions in alphaE and alphaI suppress both this effect and the constitutive activation of Gcn2 conferred by YKD Gcd- substitutions PMID: 24811037
6. Data indicate that the crystal structures of murine and yeast GCN2 protein kinase C-terminal domain (CTD) showed dimers. PMID: 24719324
7. Activation of Gcn2p by movement protein (MP) expression required a functional Tor1p kinase, since rapamycin treatment alleviated the yeast cell growth defect and blocked eIF2alpha phosphorylation triggered by MP expression PMID: 22087310
8. the interaction between Pkh1 and Gcn2 was confirmed by co-immunoprecipitation and Pkh1 was shonwn to phosphorylate Gcn2 in vitro. PMID: 22326914
9. purified eEF1A reduced the ability of Gcn2 to phosphorylate its substrate, eIF2alpha, but did not diminish Gcn2 autophosphorylation. PMID: 21849502
10. Results highlight the importance of the GCN2 eIF2 kinase pathway for maintaining metabolic homeostasis, contributing to appropriate tRNA charging and growth adaptation in response to culture conditions deficient for tryptophan and arginine. PMID: 20684782
11. structural analysis of GCN2 autoinhibition and mutational activation PMID: 15964839
12. the salt bridge interaction and dimer interface observed in the PKR structure is critical for the activity of PKR, GCN2, and PERK PMID: 17202131
13. Mutationally disrupting the hydrophobic network in various ways constitutively activates eIF2alpha phosphorylation in vivo and bypasses the requirement for a key tRNA binding motif (m2) and Thr-882 in Gcn2. PMID: 19114556
14. analysis of tRNA charging and activation of the eIF2 kinase Gcn2p PMID: 19546227

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KEGG: sce:YDR283C

STRING: 4932.YDR283C