HAG1 Antibody

Code CSB-PA858331XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HAG1 Polyclonal antibody
Uniprot No.
Target Names
HAG1
Alternative Names
HAG1 antibody; BGT antibody; GCN5 antibody; HAT1 antibody; At3g54610 antibody; T14E10.180Histone acetyltransferase GCN5 antibody; AtGCN5 antibody; EC 2.3.1.48 antibody; BIG TOP protein antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HAG1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acetylates histone H3 and ADA2 proteins in vitro. Acetylates 'Lys-14' of histone H3. Acetylation of histones gives a specific tag for epigenetic transcription activation. Operates in concert with certain DNA-binding transcriptional activators. Acts via the formation of large multiprotein complexes that modify the chromatin.
Gene References into Functions
  1. required, with ADA2b, in leaves and trichomes to couple nuclear content with cell growth and morphogenesis PMID: 29846775
  2. we find that INO can directly interact with ADA2b/PROPORZ1 (PRZ1), a transcriptional coactivator that is known to interact with the histone acetyltransferase GENERAL CONTROL NONREPRESSIBLE PROTEIN 5 (GCN5, also known as HAG1). PMID: 28971961
  3. signals from the CLV transduction pathway reach the GCN5-containing complexes in the nucleus and alter the histone acetylation status of ethylene-responsive genes. PMID: 26596766
  4. It was concluded that these data indicate that GCN5 plays a key role in the preservation of thermotolerance via versatile regulation in Arabidopsis. PMID: 26576681
  5. Data show that gcn5 mutants display defects in stamen number and arrangement at later stages. PMID: 19771450
  6. The isolation and characterization of EML, a protein that is regulated by GCN5, are reported. PMID: 17151888
  7. the gcn5 mutation seriously reduced acetylation of histone H3K14 and H3K27, whereas the T-DNA insertions of the PP2C6-6 gene enhanced the acetylation of these lysine residues PMID: 18498779
  8. GCN5 associates with a large number of active promoters, independent of the bromodomain that recognizes acetylated histone tails. However, the bromodomain is required for binding subset of promoter regions of some transcription factor genes. PMID: 18644002
  9. GCN5 is essential for root stem cell niche maintenance and acts in the PLETHORA pathway. PMID: 19376933
  10. GCN5-dependent histone acetylation interferes with the miRNA pathways by regulating microRNA production at several levels. PMID: 19436261

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Subcellular Location
Nucleus.
Protein Families
Acetyltransferase family, GCN5 subfamily
Tissue Specificity
Expressed in roots and leaves.
Database Links

KEGG: ath:AT3G54610

STRING: 3702.AT3G54610.1

UniGene: At.10490

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