GCR1 Antibody

Code CSB-PA362089XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GCR1 Polyclonal antibody
Uniprot No.
Target Names
GCR1
Alternative Names
GCR1 antibody; SIT3 antibody; YPL075W antibody; LPF10W antibody; Glycolytic genes transcriptional activator GCR1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GCR1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator required for the expression of glycolytic and ribosomal genes. Forms a transcriptional activation complex with RAP1, RAP1 providing the specific DNA-binding function and GCR1 providing the activation function. Can also bind itself to DNA to a core 5'-CTTCC-3' sequence (CT box). CT box-binding is not essential, but enhances the activation function of the RAP1-GCR1 complex in promoters that contain both DNA signals (only glycolytic genes). CT box-dependent transcriptional activation requires GCR2.
Gene References into Functions
  1. GCR1 gene generates multiple protein isoforms, which are downregulated differentially upon glucose depletion. PMID: 27153533
  2. study demonstrates that inositol pyrophosphates control the activity of the major glycolytic transcription factor GCR1 PMID: 22076377
  3. Expression of GCR1, the transcriptional activator of glycolytic enzymes in the yeast Saccharomyces cerevisiae, is positively autoregulated by Gcr1p. PMID: 15789351
  4. Gcr1 stimulates cell growth by participating in nutrient-responsive gene expression. PMID: 17124610
Subcellular Location
Nucleus.
Database Links

KEGG: sce:YPL075W

STRING: 4932.YPL075W

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