Gyc88E Antibody

Code CSB-PA810257XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Gyc88E Polyclonal antibody
Uniprot No.
Target Names
Gyc88E
Alternative Names
Gyc88E antibody; CG4154Soluble guanylate cyclase 88E antibody; EC 4.6.1.2 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Gyc88E protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Heterodimers with Gyc-89Da and Gyc-89Db are activated in response to changing oxygen concentrations, alerting flies to hypoxic environments. Under normal oxygen concentrations, oxygen binds to the heme group and results in low levels of guanylyl cyclase activity. When exposed to reduced oxygen concentrations, the oxygen dissociates from the heme group resulting in activation of the enzyme.
Gene References into Functions
  1. Gyc-88E and Gyc-89Db are co-expressed in a subset of sensory neurons where they would be ideally situated to act as oxygen sensors PMID: 15485853
  2. Mutants of NO-dependent cyclase show a specifically-marked behaviour phenotype, but on the other hand they are associated with moderate biochemical defects. PMID: 17683617
  3. biochemical properties of Gyc-88E are unique for guanylate cyclases and suggest a possible function as an oxygen sensor PMID: 18044974
Subcellular Location
Cytoplasm.
Protein Families
Adenylyl cyclase class-4/guanylyl cyclase family
Tissue Specificity
Expressed in embryos in a segmental pattern in the ventral nerve cord (VNC) and in the brain, beginning at stage 15 or 16 and continuing through to stage 1. Colocalized with Gyc-89Db in several peripheral neurons that innervate trachea, basiconical sensil
Database Links

KEGG: dme:Dmel_CG4154

STRING: 7227.FBpp0110261

UniGene: Dm.16858

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