VTC5 Antibody

Code CSB-PA880787XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VTC5 Polyclonal antibody
Uniprot No.
Target Names
VTC5
Alternative Names
VTC5 antibody; VTC2L antibody; At5g55120 antibody; MCO15.7GDP-L-galactose phosphorylase 2 antibody; EC 2.7.7.69 antibody; Protein VITAMIN C DEFECTIVE 5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VTC5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Acts as a phosphorylase rather than as a transferase. Uses preferentially GDP-L-galactose and GDP-D-glucose as substrates. Lower activity with GDP-L-fucose, very low activity with GDP-D-mannose, and no activity with UDP-D-glucose, UDP-D-galactose or ADP-D-glucose. Highly specific for inorganic phosphate as the guanylyl acceptor.
Gene References into Functions
  1. Data show that At5g55120, a homologue of GDP-L-galactose phosphorylase (VTC2), encodes a second GDP-L-galactose phosphorylase with similar properties to VTC2. PMID: 17877701
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
GDPGP1 family
Tissue Specificity
Expressed in leaves, stems, roots, flowers and siliques.
Database Links

KEGG: ath:AT5G55120

STRING: 3702.AT5G55120.1

UniGene: At.21005

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