GI Antibody

Code CSB-PA882924XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GI Polyclonal antibody
Uniprot No.
Target Names
GI
Alternative Names
GI antibody; At1g22770 antibody; T22J18.6 antibody; Protein GIGANTEA antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GI protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in regulation of circadian rhythm and photoperiodic flowering. May play a role in maintenance of circadian amplitude and period length. Is involved in phytochrome B signaling. Stabilizes ADO3 and the circadian photoreceptor ADO1/ZTL. Regulates 'CONSTANS' (CO) in the long-day flowering pathway by modulating the ADO3-dependent protein stability of CDF1 and CDF2, but is not essential to activate CO transcription. Regulates, via the microRNA miR172, a CO-independent pathway that promotes photoperiodic flowering by inducing 'FLOWERING LOCUS T'.
Gene References into Functions
  1. daily rhythms of GIGANTEA expression respond to day length in a way that depends on the latitude of origin of accessions PMID: 28644109
  2. ABA-dependent control of signalling enables drought escape via up-regulation of FLOWERING LOCUS T PMID: 27733440
  3. Data indicate three conserved regulatory modules (CRMs) within the GIGANTEA (GI) promoter. PMID: 25361953
  4. transitory regulator of SOS pathway activity whose presence or amount connects flowering to environmental conditions PMID: 23656866
  5. The drought escape response requires GI, FT/TSF, and AGL20 proteins in Arabidopsis. PMID: 23719890
  6. Spatial partitioning of GI enhances computational performance of the plant circadian network. PMID: 23830866
  7. GI expressed in either mesophyll or vascular bundles rescues the late-flowering phenotype of the gi-2 loss-of-function mutant under both short-day and long-day conditions. PMID: 21709243
  8. Soluble affinity-tagged full-length GI was expressed in Escherichia coli (E. coli. PMID: 21111047
  9. GI acts as part of the sucrose-signaling network and propose this role permits metabolic input into circadian timing in Arabidopsis. PMID: 21383174
  10. Data indicate that GI mutants gi-2 and gi-3 showed that the amount of phloem parenchyma containing wall ingrowths was reduced 15-fold compared with wild-type. PMID: 20545890
  11. The post-translational activation of GI and promotion of flowering by steroid application supports a nuclear role for GI in the floral transition. PMID: 19943973
  12. Loss of GI function causes late flowering and reduces CO and FT RNA levels PMID: 15155885
  13. These results suggest that GI gene positively regulates freezing tolerance via a CBF-independent pathway. PMID: 16231185
  14. GI plays a critical role in maintaining rhythmicity in leaf movement at higher temperatures. PMID: 16617099
  15. Plants seem to respond to changes in the environment partly through environmentally induced modifications of a basal clock-regulated pattern of Gigantea transcript accumulation PMID: 16775702
  16. GIGANTEA (GI) acts both in series with and in parallel to TOC1 within the central circadian oscillator and its function in the regulation of a clock output can be biochemically separated from its role within the circadian clock. PMID: 17098855
  17. GI affects phyA-mediated very-low-fluence responses via mechanisms that do not obviously involve its circadian functions. PMID: 17384162
  18. A significant association between GI and total fruit set that resulted in a 14% difference in average fruit set among GI haplogroups, was detected. PMID: 17614917
  19. GIGANTEA is essential to establish and sustain oscillations of ZEITLUPE by a direct protein-protein interaction PMID: 17704763
  20. FKF1 & GIGANTEA (GI) form a complex in a blue-light-dependent manner; FKF1-GI complex forms on CO promoter in late afternoon to regulate CO expression providing mechanism of how coincidence of light with circadian timing regulates photoperiodic flowering PMID: 17872410
  21. GI-mediated photoperiodic flowering is governed by the coordinated interaction of two distinct genetic pathways: one mediated via CO and the other mediated via miR172 and its targets. PMID: 17890372
  22. regulate gene expression of CO gene in arabidopsis. PMID: 18453150

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
GIGANTEA family
Tissue Specificity
Widely expressed with highest levels in inflorescence apices, young flowers and young siliques.
Database Links

KEGG: ath:AT1G22770

STRING: 3702.AT1G22770.1

UniGene: At.209

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