GIP1 Antibody

Code CSB-PA263627XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GIP1 Polyclonal antibody
Uniprot No.
Target Names
GIP1
Alternative Names
GIP1 antibody; At3g13222 antibody; GBF-interacting protein 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GIP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Plant specific protein that enhances G-box-binding factor (GBF) DNA binding activity. May function as a nuclear chaperone or lever and regulate the multimeric state of GBFs. May contribute to bZIP-mediated gene regulation. Is able to refold denatured rhodanese in vitro. Reduces DNA-binding activity of BZIP16, BZIP68 and GBF1 under non-reducing conditions through direct physical interaction. Acts as negative co-regulator in red and blue light-mediated hypocotyl elongation. Functions to promote hypocotyl elongation during the early stages of seedling development by regulating the repression effect by BZIP16 and the activation effect by BZIP68 and GBF1 on LHCB2.4 expression. Enhances transcriptional activity of LBD18 in the EXP14 promoter. May act as a transcriptional coactivator of LBD18.
Gene References into Functions
  1. the contribution of both MGO3 and GIP1 to the regulation of centromeric cohesion in Arabidopsis PMID: 28033038
  2. GIP1 functions to promote hypocotyl elongation during the early stages of Arabidopsis seedling development. PMID: 25387999
  3. ascribe a central function to GIPs for the proper recruitment and/or stabilization of centromeric proteins essential in the specification of the centromere identity, as well as for centromeric cohesion in somatic cells PMID: 26124146
  4. GIP1 functions to enhance GBF DNA binding activity by acting as a potent nuclear chaperone or crowbar, and potentially regulates the multimeric state of GBFs, thereby contributing to bZIP-mediated gene regulation. PMID: 16117846
  5. The possible role of this protein during the nucleation process is discussed PMID: 18178112

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Subcellular Location
Nucleus.
Protein Families
GIP1 family
Tissue Specificity
Expressed in roots, leaves, stems and flowers.
Database Links

KEGG: ath:AT3G13222

STRING: 3702.AT3G13222.1

UniGene: At.27156

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