GIS Antibody

Datasheet

Code	CSB-PA206615XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GIS Polyclonal antibody

Uniprot No.

Q84WI0

Target Names

GIS

Alternative Names

GIS antibody; At3g58070 antibody; T10K17.280Zinc finger protein GIS antibody; Protein GLABROUS INFLORESCENCE STEMS antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GIS protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Probable transcription factor required for the initiation of inflorescence trichomes in response to gibberellin (GA). Mediates the induction of GL1 expression by GA in inflorescence organs and is antagonized in its action by the DELLA repressor GAI. Acts upstream of the trichome initiation regulators GL1 and GL3, and downstream of the GA signaling repressor SPINDLY (SPY). Does not play a significant role in the cytokinin response. Controls trichome branching through GA signaling. Acts downstream of the key regulator STICHEL (STI) in an endoreduplication-independent pathway. Controls trichome cell division indirectly by acting downstream of a key endoreduplication regulator SIAMESE (SIM).

Gene References into Functions

GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM PMID: 23825141
GIS is required for the gibberellic acid-regulated trichome branching. GIS is not required for cell endoreduplication but functions downstream of the key regulator STI to control trichome branching in an endoreduplication-independent pathway. PMID: 22210898
show that ZFP5 encodes a protein functionally equivalent to GIS and GIS2 in controlling trichome initiation PMID: 22301962
GLABROUS INFLORESCENCE STEMS (GIS) is a putative C2H2 transcription factor which regulates several aspects of shoot maturation in Arabidopsis. [GIS] PMID: 16679458

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in inflorescence meristems, floral meristems and stem epidermis.

Database Links

KEGG: ath:AT3G58070

STRING: 3702.AT3G58070.1

UniGene: At.34767

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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