Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GL3 Polyclonal antibody

Uniprot No.

Q9FN69

Target Names

GL3

Alternative Names

GL3 antibody; BHLH1 antibody; EN31 antibody; MYC6 antibody; SST antibody; At5g41315 antibody; MYC6.2Transcription factor GLABRA 3 antibody; Basic helix-loop-helix protein 1 antibody; AtMYC6 antibody; AtbHLH1 antibody; bHLH 1 antibody; Protein SHAPESHIFTER antibody; Transcription factor EN 31 antibody; bHLH transcription factor bHLH001 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GL3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription activator, when associated with MYB75/PAP1, MYB90/PAP2 or TT2. Involved in epidermal cell fate specification. Regulates negatively stomata formation, but, in association with TTG1 and MYB0/GL1, promotes trichome formation, branching and endoreplication. Regulates also trichome cell wall maturation. Together with MYB66/WER, promotes the formation of non-hair cells in root epidermis cells in the N position. Whereas together with CPC, promotes the formation of hair cells in root epidermis cells in the H position by inhibiting non-hair cell formation. Seems also to play a role in the activation of anthocyanin biosynthesis, probably together with MYB75/PAP1. Activates the transcription of GL2.

Gene References into Functions

At1g01380 protein can inhibit the interaction between the positive trichome regulators GL1 and GL3, and likely limits trichome initiation via this inhibition. [GL3] PMID: 15584952
A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis. PMID: 14561633
Data indicate that GLABRA3 (GL3)-mediated gene expression was tissue specific. PMID: 28216162
GL3 affects the expression of UPL3. PMID: 23373825
GL3::SlGL3 transformation did not show any obvious effect on trichome or non-hair cell differentiation; tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation PMID: 23326563
A novel interaction between mutant alleles of GLABRA3 and SIAMESE, both required for differentiation, is reported. PMID: 17764505
The role of GL3 in the initiation of trichomes from pluripotent epidermal cells in Arabidopsis is reported. PMID: 17885086
Data show that GL3 associates with TRANSPARENT TESTA GLABRA1 in vivo, forming a complex. PMID: 18434419
the role of GL3 in Arabidopsis trichome formation PMID: 18547143
GL3 is a key transcription factor of wound-induced trichome formation acting downstream of jasmonic acid signaling in Arabidopsis PMID: 19234066
Anthocyanins accumulate in both wild type and egl3, but not in gl3 loss-of-function mutants when depleted of nitrogen. PMID: 19621239
GL3 and EGL3 participate in an intercellular regulatory circuit that controls cell patterning in the Arabidopsis root epidermis [GL3] PMID: 15590742

Hide All

Subcellular Location

Nucleus. Note=Moves from developing hair cells (trichoblasts) to developing non-hair cells (atrichoblasts). Also detected in trichome nucleus.

Tissue Specificity

Mostly expressed in roots and flowers. Also present in stems and leaves, and, to a lower extent, in hypocotyls. Expressed in epidermal root hair cells (trichoblasts) and moves to root hairless cells (atrichoblasts) by a cell-to-cell movement through plasm

Database Links

KEGG: ath:AT5G41315

STRING: 3702.AT5G41315.1

UniGene: At.27204

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