Recombinant Arabidopsis thaliana Transcription factor GLABRA 3 (GL3), partial

Code CSB-YP872298DOA
MSDS
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Source Yeast
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Code CSB-EP872298DOA
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Source E.coli
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Code CSB-EP872298DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872298DOA
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Source Baculovirus
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Code CSB-MP872298DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GL3
Uniprot No.
Alternative Names
GL3; BHLH1; EN31; MYC6; SST; At5g41315; MYC6.2Transcription factor GLABRA 3; Basic helix-loop-helix protein 1; AtMYC6; AtbHLH1; bHLH 1; Protein SHAPESHIFTER; Transcription factor EN 31; bHLH transcription factor bHLH001
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator, when associated with MYB75/PAP1, MYB90/PAP2 or TT2. Involved in epidermal cell fate specification. Regulates negatively stomata formation, but, in association with TTG1 and MYB0/GL1, promotes trichome formation, branching and endoreplication. Regulates also trichome cell wall maturation. Together with MYB66/WER, promotes the formation of non-hair cells in root epidermis cells in the N position. Whereas together with CPC, promotes the formation of hair cells in root epidermis cells in the H position by inhibiting non-hair cell formation. Seems also to play a role in the activation of anthocyanin biosynthesis, probably together with MYB75/PAP1. Activates the transcription of GL2.
Gene References into Functions
  1. At1g01380 protein can inhibit the interaction between the positive trichome regulators GL1 and GL3, and likely limits trichome initiation via this inhibition. [GL3] PMID: 15584952
  2. A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis. PMID: 14561633
  3. Data indicate that GLABRA3 (GL3)-mediated gene expression was tissue specific. PMID: 28216162
  4. GL3 affects the expression of UPL3. PMID: 23373825
  5. GL3::SlGL3 transformation did not show any obvious effect on trichome or non-hair cell differentiation; tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation PMID: 23326563
  6. A novel interaction between mutant alleles of GLABRA3 and SIAMESE, both required for differentiation, is reported. PMID: 17764505
  7. The role of GL3 in the initiation of trichomes from pluripotent epidermal cells in Arabidopsis is reported. PMID: 17885086
  8. Data show that GL3 associates with TRANSPARENT TESTA GLABRA1 in vivo, forming a complex. PMID: 18434419
  9. the role of GL3 in Arabidopsis trichome formation PMID: 18547143
  10. GL3 is a key transcription factor of wound-induced trichome formation acting downstream of jasmonic acid signaling in Arabidopsis PMID: 19234066
  11. Anthocyanins accumulate in both wild type and egl3, but not in gl3 loss-of-function mutants when depleted of nitrogen. PMID: 19621239
  12. GL3 and EGL3 participate in an intercellular regulatory circuit that controls cell patterning in the Arabidopsis root epidermis [GL3] PMID: 15590742

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Subcellular Location
Nucleus. Note=Moves from developing hair cells (trichoblasts) to developing non-hair cells (atrichoblasts). Also detected in trichome nucleus.
Tissue Specificity
Mostly expressed in roots and flowers. Also present in stems and leaves, and, to a lower extent, in hypocotyls. Expressed in epidermal root hair cells (trichoblasts) and moves to root hairless cells (atrichoblasts) by a cell-to-cell movement through plasm
Database Links

KEGG: ath:AT5G41315

STRING: 3702.AT5G41315.1

UniGene: At.27204

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