gld-3 Antibody

Code CSB-PA563929XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans gld-3 Polyclonal antibody
Uniprot No.
Target Names
gld-3
Alternative Names
gld-3 antibody; T07F8.3Defective in germ line development protein 3 antibody; Germline development defective 3 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans gld-3 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required maternally for germline survival and embryogenesis. Forms a complex with gls-1 which promotes the oogenic cell fate by freeing the translational repressor fbf to repress sperm promoting factors. Promotes maturation of primary spermatocytes to mature sperm. Required during hermaphrodite development to promote sperm fate, which is critical for determining the normal number of sperm. Promotion of sperm fate is at the expense of oogenesis, possibly through the negative regulation of fbf. Required during male development for the continued production of sperm and inhibition of oogenesis. Together with gld-2, promotes the transition from mitosis to meiosis.
Gene References into Functions
  1. picture that emerges from the structural and biochemical data are that GLD-3 activates GLD-2 both indirectly by stabilizing the enzyme and directly by contributing positively charged residues near the RNA-binding cleft PMID: 26124149
  2. report the 2.8-A-resolution crystal structure of a proteolytically stable fragment encompassing the KH2, KH3, KH4, and KH5 domains of C. elegans GLD-3 PMID: 20823118
  3. In germ cells, GLD-3 promotes the transition from mitosis to meiosis primarily via its interaction with GLD-2, and it promotes spermatogenesis primarily via its interaction with FBF. PMID: 15454534
Subcellular Location
Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region.
Tissue Specificity
Expressed in the germline (at protein level). In adult hermaphrodites, first detected in the transition zone (TZ), weakly expressed in the early mitotic region and in pachytene germ cells, and becomes more abundantly expressed as germ cells enter diakines
Database Links

KEGG: cel:CELE_T07F8.3

STRING: 6239.T07F8.3a

UniGene: Cel.34183

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