gld-4 Antibody

Code CSB-PA550752XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans gld-4 Polyclonal antibody
Uniprot No.
Target Names
gld-4
Alternative Names
gld-4 antibody; ZK858.1Poly(A) RNA polymerase gld-4 antibody; EC 2.7.7.19 antibody; Defective in germ line development protein 4 antibody; Germline development defective-4 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans gld-4 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. The enzymatic activity is enhanced by its interaction with gls-1. Required, together with gld-2, for early meiotic progression in male and female germ cells and for gld-1 protein accumulation in the hermaphrodite germline. In the germline, forms a complex with gls-1 which directly binds to gld-1 mRNA and prevents its degradation.
Gene References into Functions
  1. A reduced number of polysomes are detected in gld-4 mutants and GLD-4 protein co-sediments with polysomes, which together suggest that GLD-4 might stimulate or maintain translation directly. PMID: 25217583
Subcellular Location
Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region.
Tissue Specificity
Germline-specific.
Database Links

KEGG: cel:CELE_ZK858.1

STRING: 6239.ZK858.1

UniGene: Cel.16131

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