GLDH Antibody

Code CSB-PA890441XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GLDH Polyclonal antibody
Uniprot No.
Target Names
GLDH
Alternative Names
GLDH; At3g47930; T17F15.200; L-galactono-1,4-lactone dehydrogenase, mitochondrial
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GLDH protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the biosynthesis of ascorbic acid. Required for the accumulation of respiratory complex I. Uses L-galactono-1,4-lactone and L-gulono-1,4-lactone as substrates, but not D-galactono-1,4-lactone, D-gulono-1,4-lactone, L-mannono-1,4-lactone or D-galactonic acid. Also active with phenazine methosulfate and 1,4-benzoquinone as electron acceptors.
Gene References into Functions
  1. GLDH is a dual-function protein that has a second, non-enzymatic function in complex I assembly as a plant-specific assembly factor. PMID: 26520835
  2. a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates. PMID: 22378782
  3. Recombinant GALDH from Arabidopsis thaliana is inactivated by hydrogen peroxide due to selective oxidation of cysteine (Cys)-340, located in the cap domain. [GALDH] PMID: 19369590
  4. Data show that AtGALDH forms a flavin N5 sulfite adduct and that AtGALDH lacks the histidine involved in covalent FAD binding, but contains a leucine instead. PMID: 18190525
  5. L-galactono-1,4-lactone dehydrogenase is required for the accumulation of respiratory complex I PMID: 18799460

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Subcellular Location
Mitochondrion membrane; Single-pass membrane protein.
Database Links

KEGG: ath:AT3G47930

STRING: 3702.AT3G47930.1

UniGene: At.1530

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