Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GLE1 Polyclonal antibody

Uniprot No.

Q12315

Target Names

GLE1

Alternative Names

GLE1 antibody; BRR3 antibody; RSS1 antibody; YDL207W antibody; D1049 antibody; Nucleoporin GLE1 antibody; Nuclear pore protein GLE1 antibody; RNA export factor GLE1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GLE1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved in a terminal step of poly(A)+ mRNA transport through the NPC probably by binding the ATP-dependent RNA helicase DBP5 and GFD1 at the cytoplasmic side of the NPC. These interactions are thought to be important for the dissociation of transport proteins such as the heterogeneous nuclear ribonucleoprotein (hnRNP) NAB2 from exported mRNA.

Gene References into Functions

Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation. PMID: 21949122
Gle1 specifically binds IP(6); this interaction is required for the full potentiation of Dbp5 ATPase activity during both mRNA export and translation termination PMID: 20371601
Gle1, Nab2 and Gfd1 associate in a complex;Gfd1 serves as a bridging factor between Gle1 and Nab2-bound mRNA during export PMID: 15208322
the Gle1-mRNA export pathway is mediated by cytoplasmic inositol hexakisphosphate production PMID: 15459192
Dbp5 activation at NPCs requires Gle1 and InsP6. This would facilitate spatial control of the remodelling of mRNP protein composition during directional transport and provide energy to power transport cycles. PMID: 16783363
Our results define specific functions for Gle1 and InsP6 in mRNA export and suggest that local activation of Dbp5 at the nuclear pore is critical for mRNA export. PMID: 16783364
Study shows that Gle1 and inositol hexakisphosphate are required for efficient translation termination, in addition, Gle1 has a conserved physical association with the initiation factor eIF3. PMID: 18724935
Study identified several charged surface residues that, when mutated, weaken the binding of Gle1 and inhibit the ability of Gle1 to stimulate Dbp5's ATPase activity. PMID: 19805289

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Subcellular Location

Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Biased towards cytoplasmic side.

Protein Families

GLE1 family

Database Links

KEGG: sce:YDL207W

STRING: 4932.YDL207W

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GLE1 Antibody

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