GLN3 Antibody

Code CSB-PA325989XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GLN3 Polyclonal antibody
Uniprot No.
Target Names
GLN3
Alternative Names
GLN3 antibody; YER040WNitrogen regulatory protein GLN3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GLN3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.
Gene References into Functions
  1. two different ways in which intracellular Gln3 localization is regulated PMID: 26333687
  2. nuclear localization domains of GATA activator Gln3 are required for transcription of target genes, presumably through dephosphorylation PMID: 25641578
  3. Ppz1 controls Gln3 localization by regulating its phosphorylation state in combination with Sat4 and Hal5. PMID: 21237705
  4. Gln3 localization in Saccharomyces cerevisiae is separable from and can overwhelm nitrogen source regulation PMID: 17439949
  5. there is a role for Golgi-to-endosome vesicular trafficking in TORC1-controlled nuclear translocation of Gln3 PMID: 18443284

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YER040W

STRING: 4932.YER040W

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