GLB1 Antibody

Code CSB-PA946599XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GLB1 Polyclonal antibody
Uniprot No.
Target Names
GLB1
Alternative Names
GLB1 antibody; GLNB1 antibody; At4g01900 antibody; T7B11.16 antibody; Nitrogen regulatory protein P-II homolog antibody; Protein PII-like antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GLB1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Participates in sensing carbon and organic nitrogen status and regulates some steps of primary carbon and nitrogen metabolism. Required for nitrite uptake in chloroplasts and regulates arginine biosynthesis through interaction with acetylglutamate kinase (NAGK) in chloroplasts. Regulates fatty acids synthesis in chloroplasts by interacting with the acetyl-CoA carboxylase complex and inhibiting acetyl-CoA carboxylase (ACCase) activity.
Gene References into Functions
  1. role in the fine tuning of fatty acid biosynthesis and partitioning in seeds PMID: 21070409
  2. These data show that PII function has evolved between bacterial and plant systems to control the carbon metabolism pathway of fatty acid synthesis in plastids, through regulation of acetyl-CoA carboxylase activity. PMID: 20018655
  3. data support the hypothesis of a subtle involvement of the PII protein in the regulation of some steps of primary C and N metabolism PMID: 16133214
  4. Data show that the major Arabidopsis PII-interacting protein is the chloroplast-localized enzyme N-acetyl glutamate kinase, which catalyzes the key regulatory step in the pathway to arginine biosynthesis. PMID: 16377628
  5. In vitro assays using purified recombinant proteins showed the catalytic activation of Arabidopsis N-acetyl glutamate kinase (NAGK) by PII giving the first evidence of a physiological role of the PII protein in higher plants. PMID: 16545809
  6. crystal structure; a highly conserved segment at the N-terminus that is found only in plant PII forms numerous interactions with the alpha2 helix and projects from the surface of the homotrimer opposite to that occupied by the T-loop PMID: 17279613
  7. A light-dependent nitrite uptake into chloroplasts is increased in PII knock-out mutants. PMID: 18325336

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Subcellular Location
Plastid, chloroplast.
Protein Families
P(II) protein family
Database Links

KEGG: ath:AT4G01900

STRING: 3702.AT4G01900.1

UniGene: At.3850

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