glnE Antibody

Code CSB-PA512575XA01ENU
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12 / MC4100 / BW2952) glnE Polyclonal antibody
Uniprot No.
Target Names
glnE
Alternative Names
glnE antibody; BWG_2764Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme antibody; ATP:glutamine synthetase adenylyltransferase antibody; ATase) [Includes: Glutamine synthetase adenylyl-L-tyrosine phosphorylase antibody; EC 2.7.7.89 antibody; Adenylyl removase antibody; AR antibody; AT-N); Glutamine synthetase adenylyl transferase antibody; EC 2.7.7.42 antibody; Adenylyl transferase antibody; AT antibody; AT-C)] antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12 / MC4100 / BW2952)
Immunogen
Recombinant Escherichia coli (strain K12 / MC4100 / BW2952) glnE protein
Immunogen Species
Escherichia coli (strain K12 / MC4100 / BW2952)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.
Protein Families
GlnE family
Database Links

KEGG: ebw:BWG_2764

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