glpF Antibody

Code CSB-PA365183XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) glpF Polyclonal antibody
Uniprot No.
Target Names
glpF
Alternative Names
glpF; b3927; JW3898; Glycerol uptake facilitator protein; Aquaglyceroporin
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) glpF protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
Gene References into Functions
  1. Mutation of tryptophan Trp219 affects the aqualgyeroporin GlpF activity and the stability of the active GlpF tetramer. PMID: 29069569
  2. Osmotic water permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations have been presented. PMID: 28455098
  3. Folding of the aquaglyceroporin GlpF determines its stability. (Review) PMID: 25462169
  4. TMAO increases the heat stability of the GF tetramer an average of 10 degrees C in the 4 detergents and also protects the protein from denaturation by SDS. PMID: 25387032
  5. The tetrameric alpha-helical GlpF unfolds via a dimeric folding intermediate. PMID: 22035256
  6. GlpF is found to be highly permeable for small solutes and permeable for larger solutes. PMID: 18202181
  7. GlpF exists as a stably folded tetramer in detergent solutions of neutral dodecyl maltoside and in zwitterionic lysomyristoylphosphatidylcholine. PMID: 18284214

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Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
MIP/aquaporin (TC 1.A.8) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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