GLR3.3 Antibody

Code CSB-PA871686XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GLR3.3 Polyclonal antibody
Uniprot No.
Target Names
GLR3.3
Alternative Names
GLR3.3 antibody; At1g42540 antibody; T8D8.1Glutamate receptor 3.3 antibody; Ligand-gated ion channel 3.3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GLR3.3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Glutamate-gated receptor that probably acts as non-selective cation channel, at least in roots and hypocotyls (Probable). Can be triggered by Ala, Asn, Cys, Glu, Gly, Ser and glutathione (a tripeptide consisting of Glu-Gly-Cys). Mediates leaf-to-leaf wound signaling. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells. Contributes to pathogen-associated molecular patterns (PAMP) elicitor-mediated resistance. Partially involved in free cytosolic calcium variations, nitric oxide (NO) production, reactive oxygen species (ROS) production and expression of defense-related genes in response to oligogalacturonide elicitors. Inovlved in resistance against Hyaloperonospora arabidopsidis. Required for glutathione-induced defense responses, and innate immunity responses against the bacterial pathogen Pseudomonas syringae pv tomato strain DC3000. Required for the transmission of wound-induced, phloem-propagated action potential to neighbor leaves.
Gene References into Functions
  1. GLR3.3 is a key component of resistance against Hyaloperonospora arabidopsidis PMID: 23952652
  2. AtGLR3.3 is required for glutathione-mediated innate immunity response in Arabidopsis Leaf. PMID: 23656893
  3. Six amino acids commonly present in soils as well as the tripeptide glutathione were found to be strong agonists of GLR3.3-mediated calcium metabolism. PMID: 17012403
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Glutamate-gated ion channel (TC 1.A.10.1) family
Tissue Specificity
Expressed predominantly in roots and siliques.
Database Links

KEGG: ath:AT1G42540

STRING: 3702.AT1G42540.1

UniGene: At.39090

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