Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRX1 Polyclonal antibody

Uniprot No.

P25373

Target Names

GRX1

Alternative Names

GRX1 antibody; YCL035C antibody; YCL35CGlutaredoxin-1 antibody; EC 1.11.1.9 antibody; EC 2.5.1.18 antibody; Glutathione-dependent oxidoreductase 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRX1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the glutathione system which performs several activities such as glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing cytosolic protein- and non-protein-disulfides in a coupled system with glutathione reductase. Required for resistance to reactive oxygen species (ROS) by directly reducing hydroperoxides and for the detoxification of ROS-mediated damage. GRX1 is less active as an oxidoreductase than GRX2.

Gene References into Functions

Grx1p and Grx2p protect against Selenite-induced cell death. PMID: 20522499
analysis of the structural basis for the different activities of yeast Grx1 and Grx2 PMID: 20417731
Redox-sensitive yellow fluorescent protein fused to glutaredoxin 1 (Grx1p). Intramolecular transfer of reducing equivalents made the fusion protein kinetically superior in reactions with glutathione. PMID: 16475825
Glutaredoxin Grx1p has been crystallized both as an independent protein and in a protein fusion with histidine-tagged yellow fluorescent protein. PMID: 16946480
Comparative structural analyses of the oxidized and glutathionylated Grx1 illustrate the conformational changes of residues at different redox states, whose roles during catalysis are further compared with the homologous structures. PMID: 18473363
We hypothesize that the substitutions of Ser23 and Gln52 in yGrx1 by Ala23 and Glu52 in yGrx2 modify the capability of the active-site C-terminal cysteine to attack the mixed disulfide between the N-terminal active-site cysteine and glutathione molecule PMID: 18992757

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Glutaredoxin family

Database Links

KEGG: sce:YCL035C

STRING: 4932.YCL035C

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GRX1 Antibody

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