GRX3 Antibody

Code CSB-PA312741XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRX3 Polyclonal antibody
Uniprot No.
Target Names
GRX3
Alternative Names
GRX3 antibody; YDR098C antibody; YD8557.05CMonothiol glutaredoxin-3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRX3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit (Probable).
Gene References into Functions
  1. Slt2 form iron/sulphur bridged clusters with Grx3 and Grx4. PMID: 28007574
  2. This article reports mechanistic studies that investigate the role of exogenous glutathione in defining cluster chirality, ligand exchange, and the cluster transfer chemistry of Saccharomyces cerevisiae Grx3. PMID: 28836015
  3. RNR cofactor biogenesis requires the ISC machinery to mature the Grx3/4 and Dre2 Fe-S proteins, which then function in iron and electron delivery to RNR, respectively. PMID: 28515324
  4. diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.( PMID: 21931161
  5. The authors present data showing that both Grx3 and Grx4 have roles in actin cytoskeleton remodeling and in cellular defenses against oxidative stress caused by reactive oxygen species (ROS) accumulation. PMID: 20889785
  6. Grx3 and Grx4 are novel components required for Aft1 iron regulation that most likely occurs in the nucleus PMID: 16648636
  7. crystal structure of the thioredoxin-like domain determined at 1.5 A resolution. loop containing signature motif WAxxC is partially disordered, indicating greater flexibility compared with classical dithiol thioredoxins with a WCGPC active-site motif. PMID: 18703840
  8. The ability of the Fra2-Grx3/4 complex to assemble a [2Fe-2S] cluster may act as a signal to control the iron regulon in response to cellular iron status in yeast. PMID: 19715344

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Protein Families
Glutaredoxin family, Monothiol subfamily
Database Links

KEGG: sce:YDR098C

STRING: 4932.YDR098C

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