GRX5 Antibody

Code CSB-PA877771XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRX5 Polyclonal antibody
Uniprot No.
Target Names
GRX5
Alternative Names
GRX5 antibody; YPL059W antibody; LPE13WMonothiol glutaredoxin-5 antibody; mitochondrial antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRX5 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer. Receives 2Fe/2S clusters from scaffold protein ISU1 and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion.
Gene References into Functions
  1. Potential roles for monothiol Grx5 in scavenging and recycling linear [Fe3S4](+) clusters released during protein unfolding under oxidative stress conditions and in maturation of [Fe4S4](2+) cluster-containing proteins PMID: 24032439
  2. Grx5 acts on iron-sulfur cluster biosynthesis by regulating the redox state of important cysteine residues in Nfs1 and/or in the scaffold proteins where iron-sulfur clusters initially assemble PMID: 15382238
  3. Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5 are reported. PMID: 19478456
Subcellular Location
Mitochondrion matrix.
Protein Families
Glutaredoxin family, Monothiol subfamily
Database Links

KEGG: sce:YPL059W

STRING: 4932.YPL059W

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