GLU1 Antibody

Code CSB-PA831610XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GLU1 Polyclonal antibody
Uniprot No.
Target Names
GLU1
Alternative Names
GLU1 antibody; GLS1 antibody; At5g04140 antibody; F21E1_60Ferredoxin-dependent glutamate synthase 1 antibody; chloroplastic/mitochondrial antibody; EC 1.4.7.1 antibody; Fd-GOGAT 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GLU1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Involved in glutamate biosynthesis in leaf. Required for the reassimilation of ammonium ions generated during photorespiration.
Gene References into Functions
  1. glu1-2 mutant transcriptome was characterised by an extensive secondary response including the downregulation of photosynthesis-related pathways and the induction of genes and pathways involved in the plant response to a multitude of stresses. PMID: 20307264
  2. Expression of GLU1 in mesophyll cells implies that Fd-GOGAT assimilates photorespiratory and primary ammonium. GLU1 expression in vascular cells indicates that Fd-GOGAT provides amino acids for nitrogen translocation. PMID: 16034598
  3. GLU1 interacts with SHMT1 and this interaction is necessary for photorespiratory serine hydroxymethyltransferase (SHMT) activity. PMID: 19223513
Subcellular Location
Plastid, chloroplast stroma. Mitochondrion matrix.
Protein Families
Glutamate synthase family
Tissue Specificity
Highly expressed in leaves. High expression in the leaf mesophyll and phloem companion cell-sieve element complex.
Database Links

KEGG: ath:AT5G04140

STRING: 3702.AT5G04140.2

UniGene: At.21961

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