glyA Antibody

Datasheet

Code	CSB-PA021274XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) glyA Polyclonal antibody

Uniprot No.

P0A825

Target Names

glyA

Alternative Names

glyA antibody; b2551 antibody; JW2535Serine hydroxymethyltransferase antibody; SHMT antibody; Serine methylase antibody; EC 2.1.2.1 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) glyA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of allothreonine and 3-phenylserine. Also catalyzes the irreversible conversion of 5,10-methenyltetrahydrofolate to 5-formyltetrahydrofolate.

Gene References into Functions

A bioinformatic study shows that Tyr55 (at the active site) and Arg235 are strongly conserved within the serine hydroxymethyltransferase family and are involved in a cation-pi interaction in the large majority of available SHMT structures. PMID: 19883126
activation of glyA by CsgD requires the participation of the regulatory proteins, MetR and PurR PMID: 15644921
Results describe the mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase. PMID: 17341210

Subcellular Location

Cytoplasm.

Protein Families

SHMT family

Database Links

KEGG: ecj:JW2535

STRING: 316385.ECDH10B_2718

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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glyA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background