GNL1 Antibody

Code CSB-PA264913XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GNL1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
GNL1 antibody; ERMO1 antibody; GBF1 antibody; At5g39500 antibody; MUL8.20ARF guanine-nucleotide exchange factor GNL1 antibody; Protein ENDOPLASMIC RETICULUM MORPHOLOGY 1 antibody; Protein GNOM-like 1 antibody
Raised in
Species Reactivity
Arabidopsis thaliana
Recombinant Arabidopsis thaliana GNL1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Purification Method
Antigen Affinity Purified
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Activates the ARF proteins by exchanging bound GDP for free GTP. Plays a role in vesicular protein sorting. Acts as the major regulator of retrograde Golgi to endoplasmic reticulum trafficking but is also involved in the endocytosis process. Could function redundantly with GNOM. Regulates vesicle trafficking required for the coordinated polar localization of auxin efflux carriers which in turn determines the direction of auxin flow. Mediates the endocytosis of PIN2 from plasma membrane to endosomal compartments. Required for maintenance of endoplasmic reticulum morphology.
Gene References into Functions
  1. These data reveal an unanticipated level of complexity in the biology of GNL1 by showing that its cellular roles are correlated with cell growth. PMID: 23125314
  2. Large ARF-GEFs of the GBF1 class perform a conserved role in endoplasmic reticulum-Golgi trafficking and secretion, which is done by GNL1 and GNOM in Arabidopsis. [GNL1] PMID: 17653190
  3. GNL1 is a BFA-resistant GBF protein that functions with a BFA-sensitive ARF-GEF both at the Golgi and in selective endocytosis, but not in recycling from endosomes. [GNL1] PMID: 17653191
  4. GNL2 encodes a protein homologous to the adenosine diphosphate-ribosylation factor-guanine nucleotide exchange factors, GNOM and GNL1 that are involved in endosomal recycling and endoplasmic reticulum-Golgi vesicular trafficking PMID: 19686373
Subcellular Location
Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Soluble and partially membrane-bound.
Database Links

KEGG: ath:AT5G39500

STRING: 3702.AT5G39500.1

UniGene: At.30340

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