sau Antibody

Code CSB-PA282005XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) sau Polyclonal antibody
Uniprot No.
Target Names
sau
Alternative Names
sau antibody; GOLPH3 antibody; l(2)s5379 antibody; rti antibody; CG7085Golgi phosphoprotein 3 homolog sauron antibody; Protein rotini antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) sau protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Also involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. Functions in cytokinesis by regulating contractile ring formation and vesicle trafficking during cleavage furrow ingression. May also have a role in the intital steps of central spindle formation. Can also bind phosphatidylinositol-3-phosphate and phosphatidylinositol-5-phosphate in vitro.
Gene References into Functions
  1. It has been demonstrated that the COG complex cooperates with Rab1 and GOLPH3 to regulate Golgi trafficking, and that overexpression of Rab1 can rescue the cytokinesis and locomotor defects associated with loss of Cog7. PMID: 28883096
  2. Study provides evidence that Rab1, by associating with GOLPH3, controls membrane trafficking and contractile ring constriction during cytokinesis. PMID: 28100664
  3. Because cytokinesis failures have been associated with premalignant disease and cancer, our studies suggest novel insight into molecular circuits involving the oncogene GOLPH3 in cytokinesis PMID: 24786584
Subcellular Location
Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle. Cleavage furrow.
Protein Families
GOLPH3/VPS74 family
Database Links

KEGG: dme:Dmel_CG7085

STRING: 7227.FBpp0100140

UniGene: Dm.20470

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