GOLS1 Antibody

Code CSB-PA191518XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GOLS1 Polyclonal antibody
Uniprot No.
Target Names
GOLS1
Alternative Names
GOLS1 antibody; At2g47180 antibody; T3D7 antibody; T8I13Galactinol synthase 1 antibody; AtGolS1 antibody; GolS-1 antibody; EC 2.4.1.123 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GOLS1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Galactinol synthase involved in the biosynthesis of raffinose family oligosaccharides (RFOs) that function as osmoprotectants. Promotes plant stress tolerance such as heat, chilling, salinity and methylviologen (MV), a superoxide radical generating drug, by mediating raffinose accumulation, an osmoprotective substance.
Gene References into Functions
  1. Overexpression of AtGolS and CsRFS antagonizes the defense response to poplar leaf rust disease through repressing reactive oxygen species and attenuating calcium and phosphatidic acid signaling events that lead to SA defense. PMID: 28981890
  2. Expression of AtGOLS1 decreased when conditions were favorable for seed germination. Seed germination rate was faster in T-DNA knockout atgols1 mutant and transgenic plants transformed with an RNA interference construct targeting AtGOLS1 compared with wild type plants. PMID: 29362103
  3. Findings provide evidence that GolS1 and GolS2 are directly regulated by HsfA3 and that GolS enzymes play an important role in improving oxidative stress tolerance by increasing galactinol biosynthesis in Arabidopsis. PMID: 27109422
  4. GolS1 is a novel heat shock factor target gene, which is responsible for heat stress-dependent synthesis of raffinose in Arabidopsis. [GolS1] PMID: 15466240
Subcellular Location
Cytoplasm.
Protein Families
Glycosyltransferase 8 family, Galactosyltransferase subfamily
Tissue Specificity
Accumulates in mature seeds. Expressed in seedlings (axes and cotyledons), meristems, vascular tissues and emerging lateral roots. Present in abscission zones.
Database Links

KEGG: ath:AT2G47180

STRING: 3702.AT2G47180.1

UniGene: At.12215

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