GORK Antibody

Code CSB-PA836002XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GORK Polyclonal antibody
Uniprot No.
Target Names
GORK
Alternative Names
GORK antibody; At5g37500 antibody; MPA22.4 antibody; Potassium channel GORK antibody; Guard cell outward rectifying K(+) channel antibody; AtGORK antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GORK protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Major selective outward-rectifying potassium channel of the guard cell membrane. Involved in regulation of stomatal movements according to the water status. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by depolarization. Conductance of the channel is modulated in a potassium-dependent fashion. May interact with the cytoskeleton or with regulatory proteins.
Gene References into Functions
  1. Plants lacking functional GORK channels are more capable of retaining K+ for their better performance under hypoxia, and that RBOHD is crucial in hypoxia-induced Ca2+ signalling for stress sensing and acclimation mechanism. PMID: 28338729
  2. GORK is regulated by CPK33 PMID: 28543075
  3. GORK clustering in puncta is related to its gating and conductance, and reflects associated conformational changes and (de)stabilisation of the channel protein. PMID: 24517091
  4. Sustained activation of K+ outward current could be the result of an unexpected post-transcriptional regulation of the guard cell outward-rectifying K+ (GORK) channels. PMID: 23517047
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Potassium channel family, Plant (TC 1.A.1.4) subfamily
Tissue Specificity
Expressed in guard cell-containing tissues, in root epidermal cells and in root hairs. Detected in vascular cells of the root and shoot.
Database Links

KEGG: ath:AT5G37500

STRING: 3702.AT5G37500.1

UniGene: At.10193

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