GPD1 Antibody

Code CSB-PA311439XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GPD1 Polyclonal antibody
Uniprot No.
Target Names
GPD1
Alternative Names
GPD1 antibody; DAR1 antibody; HOR1 antibody; OSG1 antibody; YDL022W antibody; D2830Glycerol-3-phosphate dehydrogenase [NAD(+)] 1 antibody; EC 1.1.1.8 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GPD1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the production and accumulation of glycerol during hyperosmotic stress conditions. Glycerol acts as a osmoregulator that prevents loss of water and turgor of the cells.
Gene References into Functions
  1. High expression of SIP18 at the beginning of rehydration was shown to be due to high transcription of the gene during the drying process. PMID: 28600909
  2. PUB1 mutant shows increased glycerol-3-phosphate dehydrogenase activity during wine making, which is related with an increase in the Gpd1 levels. PMID: 26846624
  3. Stress exposure results in increased peroxisomal levels of yeast Pnc1 and Gpd1, which are imported via a piggy-backing mechanism. PMID: 26516056
  4. GPD1 silencing gave 20 % less glycerol-3-phosphate dehydrogenase activity, 19 % lower glycerol production, and 9.7 % higher ethanol production compared with the original strain. PMID: 24150518
  5. The crystal structure of a glycerol-3-phosphate dehydrogenase, GPD1, from S. cerevisiae. PMID: 23143232
  6. Phosphorylation of Gpd1 and Gpd2 by distinct kinases enables rapid adaptation to specific stress conditions in Saccharomyces cerevisiae. PMID: 22988299

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Subcellular Location
Cytoplasm. Peroxisome.
Protein Families
NAD-dependent glycerol-3-phosphate dehydrogenase family
Database Links

KEGG: sce:YDL022W

STRING: 4932.YDL022W

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