Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) gper1 Polyclonal antibody

Uniprot No.

B3G515

Target Names

gper1

Alternative Names

gper1 antibody; G-protein coupled estrogen receptor 1 antibody; G protein-coupled estrogen receptor 1 antibody; G-protein coupled receptor 30 antibody

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) gper1 protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Membrane G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Plays a role in the embryonic development of sensory and motor neurons. Specifically induces apoptosis and reduces proliferation of brain cells. Involved in maintenance of meiotic arrest in oocytes.

Gene References into Functions

Nppc through Npr2 cooperates with E2 through Gper in upregulation of cGMP levels to inhibit phosphodiesterase activity resulting in maintenance of oocyte meiotic arrest in zebrafish. PMID: 29574150
Study finds that GPER is required for normal heart rate in zebrafish embryos. PMID: 29065151
the potential role of the G-protein estrogen receptor 1 (GPER) in Estradiol-induced developmental toxicity, was investigated. PMID: 28284154
Data indicate that G-protein-coupled estrogen receptor 1 (Gper) can maintain the survival of the brain cells, and formation and/or differentiation of the sensory and motor neurons. PMID: 23583372
results suggest that estrogens act through Gper to maintain meiotic arrest via an Src kinase-dependent G-protein betagamma subunit signaling pathway involving transactivation of egfr and phosphorylation of Mapk3/1 PMID: 21349822
The results suggest that endogenous estrogens produced by the follicle cells inhibit or delay spontaneous maturation of zebrafish oocytes and that this estrogen action is mediated through GPER. PMID: 20382141
localization in specific brain regions and in early germ cells of the testis, including the spermatogonia, spermatocytes, and somatic cells such as Sertoli cells PMID: 19228597

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Subcellular Location

Nucleus. Cytoplasm, perinuclear region. Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Early endosome. Recycling endosome. Golgi apparatus, trans-Golgi network. Golgi apparatus membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, dendritic spine membrane; Multi-pass membrane protein. Cell projection, axon. Cell junction, synapse, postsynaptic density. Mitochondrion membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

Expressed in brain regions that are known to control reproduction and sex behavior. Expressed in ovary, muscle and intestine. Expressed in early germ cells of the testis, including the spermatogonia, spermatocytes, and somatic cells such as Sertoli cells.

Database Links

KEGG: dre:565271

STRING: 7955.ENSDARP00000070486

UniGene: Dr.148317

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gper1 Antibody

Product Details

Customer Reviews and Q&A

Target Background