NPA3 Antibody

Code CSB-PA341669XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NPA3 Polyclonal antibody
Uniprot No.
Target Names
NPA3
Alternative Names
NPA3 antibody; EPA1 antibody; GPN1 antibody; YJR072C antibody; J1821GPN-loop GTPase 1 antibody; EC 3.6.5.- antibody; Essential PCL1-interacting ATPase 1 antibody; GPN-loop GTPase NPA3 antibody; Nucleolar preribosomal-associated protein 3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NPA3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import. Promotes sister chromatid separation during anaphase.
Gene References into Functions
  1. Npa3/Gpn1 carboxy-terminal tail is dispensable for cell viability and RNA polymerase II nuclear targeting but critical for microtubule stability and function. PMID: 27965115
  2. Npa3 has chaperone activity and interacts with hydrophobic peptide regions of RNA Polymerase II subunits that form interfaces in the assembled RNA Polymerase II complex. PMID: 26711263
  3. Npa3 defines an unconventional pathway for nuclear import of RNAPII, which involves GTP-dependent binding of Npa3 to the polymerase. PMID: 21844196
Subcellular Location
Cytoplasm.
Protein Families
GPN-loop GTPase family
Database Links

KEGG: sce:YJR072C

STRING: 4932.YJR072C

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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