Gr5a Antibody

Code CSB-PA896191XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Gr5a Polyclonal antibody
Uniprot No.
Target Names
Gr5a
Alternative Names
Gr5a; GRLU.7; Tre; CG15779; Gustatory receptor 5a for trehalose; Trehalose receptor
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Gr5a protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Gustatory receptor required for response to the sugar trehalose in taste neurons. Gr5a neurons selectively respond to sugars, in contrast to Gr66a cells which respond to bitter compounds. Flies are attracted to sugars and avoid bitter substances, suggesting that Gr5a neuron activity is sufficient to mediate acceptance behavior. Sugar signal transduction occurs through coupling with G-proteins such as Galpha49B and G-salpha60A.
Gene References into Functions
  1. The detection of sugar via Gr5a gustatory receptor neurons is necessary for food-promoted male aggression. PMID: 25162609
  2. necessary for normal trehalose detection PMID: 19174988
  3. expressed in neurons of taste sensilla located on the labellum and legs PMID: 14523229
  4. Causes of the observed pattern of nucleotide variation in the gustatory receptor Gr5a gene. PMID: 15342513
  5. analysis of trehalose sensitivity of the gustatory receptor neurons expressing wild-type, mutant and ectopic Gr5a in Drosophila PMID: 15738154
  6. single P-element insertions in the intergenic region between Gr5a and Tre1 exert complex pleiotropic effects on fitness traits, including selective nutrient intake, life span, and resistance to starvation and heat stress PMID: 16783380
  7. Gustator receptors would be candidate sugar receptors because Gr5a-expressing cells function in the responses to attractive tastants. PMID: 17715294
  8. The simplest interpretation of our results is that Gr5a and Gr64a are each capable of functioning independently of each other within individual sugar neurons and that they are the primary receptors used in the labellum to detect sugars. PMID: 17988633

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Insect chemoreceptor superfamily, Gustatory receptor (GR) family, Gr5a subfamily
Tissue Specificity
Expressed in labellar chemosensory neurons.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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