Product Details

Full Product Name

Rabbit anti-Oryza sativa subsp. japonica (Rice) GRF4 Polyclonal antibody

Uniprot No.

Q6ZIK5

Target Names

GRF4

Alternative Names

GRF4 antibody; GL2 antibody; GLW2 antibody; GS2 antibody; PT2 antibody; Os02g0701300 antibody; LOC_Os02g47280 antibody; OJ1111_E07.19 antibody; OsJ_08054Growth-regulating factor 4 antibody; OsGRF4 antibody; Protein GRAIN LENGTH AND WIDTH 2 antibody; Protein GRAIN SIZE ON CHROMOSOME 2 antibody; Protein PANICLE TRAITS 2 antibody; Transcription activator GRF4 antibody

Raised in

Rabbit

Species Reactivity

Oryza sativa subsp. japonica (Rice)

Immunogen

Recombinant Oryza sativa subsp. japonica (Rice) GRF4 protein

Immunogen Species

Oryza sativa subsp. japonica (Rice)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription activator that plays a role in the regulation of meristematic function in leaves, stems and inflorescences. Transcription activator that plays a regulatory role in grain development. Positively regulates grain size by promoting cell division and expansion, leading to increased grain length and width. Positively regulates the expression of genes promoting cell proliferation. Activates the expression of expansin genes to promote cell expansion and grain size. May promote grain size by activating brassinosteroid responses. Component of a network formed by the microRNA396 (miRNA396), the GRFs and their interacting factors (GIFs) acting in the regulation of meristem function, at least partially through the control of cell proliferation (Probable). Component of the miRNA396c-GRF4-GIF1 regulatory module that plays an important role in grain size determination (Probable).

Gene References into Functions

Transcriptome profiling of 7-cm-long young panicles of NIL-Osgrf4 and NIL-OsGRF4 using RNAs sequence analyses identified 80 differentially expressed genes. The pleiotropic target gene OsGRF4 was up-regulated in NIL-OsGRF4 plants. It may also be involve in abiotic stress resistance. PMID: 29924832
GRF4 role in the grain size and yield, and also brassinosteroid metabolism PMID: 27250747
GRF4 regulation by Mir396 controls the grain size and yield. PMID: 27250749

Subcellular Location

Nucleus.

Protein Families

GRF family

Tissue Specificity

Expressed in stems. Expressed in panicles.

Database Links

KEGG: osa:4330436

UniGene: Os.49748

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GRF4 Antibody

Product Details

Customer Reviews and Q&A

Target Background