GSH1 Antibody

Code CSB-PA343567XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GSH1 Polyclonal antibody
Uniprot No.
Target Names
GSH1
Alternative Names
GSH1 antibody; CAD2 antibody; GCL antibody; PAD2 antibody; RML1 antibody; At4g23100 antibody; F7H19.290 antibody; Glutamate--cysteine ligase antibody; chloroplastic antibody; EC 6.3.2.2 antibody; Gamma-ECS antibody; GCS antibody; Gamma-glutamylcysteine synthetase antibody; Protein ROOT MERISTEMLESS 1 antibody; AtGCL antibody; Protein cadmium-sensitive 2 antibody; Protein phytoalexin-deficient 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GSH1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Seems to play an important role in controlling the expression of resistance responses like the regulation of salicylic acid (SA) and phytoalexin (camalexin) production. Involved in resistance to fungal and bacterial pathogens. Required for the regulation of cell proliferation in root apical meristems through the GSH-dependent developmental pathway. Also participates in the detoxification process, the antioxidant response and is essential for embryo development and proper seed maturation.
Gene References into Functions
  1. The pad2-1 mutation is related to a decrease in GCL protein and that the resulting glutathione deficiency negatively affects important processes of disease resistance. PMID: 22007023
  2. Glutathione is a key regulator of GSH1 gene expression in response to low temperature. PMID: 22628560
  3. GSH1 is involved in the biosynthesis of glutathione. The zir1 mutant is defective in Fe-mediated Zn tolerance. PMID: 22066515
  4. The thiol-based regulation of GCL provides a posttranslational mechanism for modulating enzyme activity in response to in vivo redox environment and suggests a role for oxidative signaling in the maintenance of glutathione homeostasis in plants. PMID: 17766407
Subcellular Location
Plastid, chloroplast.
Protein Families
Carboxylate-amine ligase family, Glutamate--cysteine ligase type 2 subfamily
Tissue Specificity
Abundant in leaves and roots. Expressed to a high level in leaf trichomes of mature plant.
Database Links

KEGG: ath:AT4G23100

STRING: 3702.AT4G23100.1

UniGene: At.25074

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