gss Antibody

Code CSB-PA365188XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) gss Polyclonal antibody
Uniprot No.
Target Names
gss
Alternative Names
gss antibody; gsp antibody; b2988 antibody; JW2956 antibody; Bifunctional glutathionylspermidine synthetase/amidase antibody; GspSA) [Includes: Glutathionylspermidine amidase antibody; Gsp amidase antibody; EC 3.5.1.78 antibody; Glutathionylspermidine amidohydrolase [spermidine-forming]); Glutathionylspermidine synthetase antibody; Gsp synthetase antibody; EC 6.3.1.8 antibody; Glutathione:spermidine ligase [ADP-forming] antibody; Gsp synthase)] antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) gss protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the formation of an amide bond between glutathione (GSH) and spermidine coupled with hydrolysis of ATP; also catalyzes the opposing reaction, i.e. the hydrolysis of glutathionylspermidine (Gsp) back to glutathione and spermidine. The amidase active site can also hydrolyze Gsp-disulfide (Gsp-S-S-Gsp) to Gsp-SG and Gsp S-thiolated proteins (GspSSPs) to GSH S-thiolated protein (GSSPs). Likely acts synergistically with glutaredoxin to regulate the redox environment of E.coli and defend against oxidative damage. In vitro, the amidase active site also catalyzes hydrolysis of amide and ester derivatives of glutathione (e.g. glutathione ethyl ester and glutathione amide) but lacks activity toward acetylspermidine (N1 and N8) and acetylspermine (N1).
Gene References into Functions
  1. Results describe two crystal structures of glutathionylspermidine amidase, GspA and GspSA, both with the C59A mutation and bound with the substrate, Gsp. PMID: 21226054
  2. The hypersensitivities of GspSA and GspSA/glutaredoxin null mutants to H(2)O(2) support the idea that GspSA and glutaredoxin act synergistically to regulate the redox environment of E. coli. PMID: 20530482
  3. Five crystal structures of Escherichia coli GspS in complex with substrate, product or inhibitor, are reported. PMID: 17124497
Protein Families
Glutathionylspermidine synthase preATP-grasp family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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