HTA1 Antibody

Code CSB-PA361409XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HTA1 Polyclonal antibody
Uniprot No.
Target Names
HTA1
Alternative Names
HTA1 antibody; H2A1 antibody; SPT11 antibody; YDR225W antibody; YD9934.10 antibody; Histone H2A.1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HTA1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene References into Functions
  1. Robust preinitiation complex-dependent H2A.Z eviction was observed at active and infrequently transcribed genes, indicating that constitutive H2A.Z turnover is a general phenomenon. PMID: 27438412
  2. Dual function of Swc5 in SWR remodeling ATPase activation and histone H2A eviction has been reported. PMID: 28973436
  3. Data indicate that copy number variation (CNV) is regulated by both promoter activity and acetylation of histone H3 lysine 56 (H3K56ac) and that H3K56ac is required for copper-resistance gene CUP1 CNV and efficient copper adaptation. PMID: 28654659
  4. The authors found that SWR1 primarily recognizes key residues within the alpha2 helix in the histone-fold of nucleosomal histone H2A, a region not previously known to influence remodeler activity. PMID: 26116819
  5. histone H2A Ser122 facilitates DNA repair PMID: 15781691

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H2A family
Database Links

KEGG: sce:YDR225W

STRING: 4932.YDR225W

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