HHT1 Antibody

Code CSB-PA352255XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HHT1 Polyclonal antibody
Uniprot No.
Target Names
HHT1
Alternative Names
HHT1 antibody; YBR010W antibody; YBR0201; antibody; HHT2 antibody; SIN2 antibody; YNL031C antibody; N2749 antibody; Histone H3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HHT1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Component of the UAF (upstream activation factor) complex which interacts with the upstream element of the RNA polymerase I promoter and forms a stable preinitiation complex. Together with SPT15/TBP, UAF seems to stimulate basal transcription to a fully activated level.
Gene References into Functions
  1. A model has been proposed wherein combined actions of Set1 and Jhd2 via modulating H3K4 methylation-demethylation together control chromatin dynamics during various facets of transcriptional regulation. PMID: 27325136
  2. Data indicate histone post-translational modifications (PTMs) enriched at a DNA double-strand break (DSB) site. PMID: 29036368
  3. The mutant ion at Histone H3 doesn't affect the recruitment of Pol II repressor RENT (regulator of nucleolar silencing and telophase exit) complex at the Ribosomal DNA region. PMID: 26906758
  4. results suggest that divergent amino acids within the histone H3 alpha3 helix play organismal roles in defining chromatin structure PMID: 26534951
  5. Data show that vacuolar protein Prb1 is present in the cellular fraction enriched for the H3 N-terminus endopeptidase activity and this endopeptidase activity is lost in the PRB1 deletion mutant (prb1Delta). PMID: 24587380
  6. The molecular mechanism linking double strand break sequences to chromosome axes and the importance of H3K4 methylation for meiotic recombination. PMID: 23246437
  7. Studies identified mutations altering eight histone residues (H3 residues V46, R49, V117, Q120, and K122 and H4 residues R36, I46, and S47) that strongly increase SER3 expression without reducing the transcription of the intergenic SRG1 ncDNA. PMID: 21730290
  8. Data identify and characterize H3-T45 phosphorylation as a replication-associated histone modification in budding yeast. PMID: 20139971
  9. Histone H3 K56 acetylation at the entry-exit gate enables recruitment of the SWI/SNF nucleosome remodeling complex and so regulates gene activity. PMID: 15882620
  10. Investigation of kinetochore assembly in the absence of the centromeric histone H3 variant Cse4. PMID: 16207811
  11. Hst3 Is regulated by Mec1-dependent proteolysis and controls the S phase checkpoint and sister chromatid cohesion by deacetylating histone H3 at lysine 56 PMID: 17977840
  12. analysis of the molecular mechanism of the recognition and binding of the methylated H3K36 by Eaf3 provides new insights into the functional roles of the Eaf3 chromo domain PMID: 18984594
  13. Collectively, these results indicate that different histone H3 Arg2 methylation states have defined roles in gene expression. PMID: 19270702

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H3 family
Database Links

KEGG: sce:YBR010W

STRING: 4932.YNL031C

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