HHF1 Antibody

1. The authors show that yeast chromatin assembly factor 1 binding to a histone H3-H4 dimer activates the Cac1 winged helix domain interaction with DNA. This drives the formation of a transient intermediate containing two chromatin assembly factor 1 complexes, each associated with one H3-H4 dimer. Here, the (H3-H4)2 tetramer is formed and deposited onto DNA. PMID: 28315523
2. Here, the authors report that yeast chromatin assembly factor 1 (CAF1), a conserved histone chaperone complex that deposits H3-H4 during DNA replication, binds a single H3-H4 heterodimer in solution. PMID: 28315525
3. Here the authors mapped the H4-tail binding pocket of ISWI. Surprisingly the binding site was adjacent to but not overlapping with the docking site of an auto-regulatory motif, AutoN, in the N-terminal region (NTR) of ISWI, indicating that AutoN does not act as a simple pseudosubstrate as suggested previously. PMID: 28109157
4. The NuA4 core complex acetylates nucleosomal histone H4 through a double recognition mechanism. PMID: 27594449
5. Here, we find that Sas2-dependent acetylated histone H4 K16Ac in bulk histones requires passage of the cells through the S-phase of the cell cycle, and the rate of increase in H4 K16Ac depends on both CAF-I and Asf1. PMID: 26260510
6. Acetylated H4K16 has a dual role in silencing: it recruits Sir2-4 and repels Sir3. Moreover, the deacetylation of H4K16(ac) by Sir2 actively promotes the high-affinity binding of the SIR holocomplex. PMID: 21666601
7. Histone H4 deposition defects lead to the formation of recombinogenic DNA structures during replication that increase genomic instability. PMID: 15684401

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KEGG: sce:YBR009C

STRING: 4932.YNL030W