PAS2 Antibody

Code CSB-PA823812XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PAS2 Polyclonal antibody
Uniprot No.
Target Names
PAS2
Alternative Names
PAS2; PEP; At5g10480; F12B17.170; Very-long-chain; 3R-3-hydroxyacyl-CoA dehydratase PASTICCINO 2; 3-hydroxyacyl-CoA dehydratase PASTICCINO 2; AtPAS2; HACD; HCD; Protein PEPINO; Protein tyrosine phosphatase-like protein
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PAS2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May be an anti-phosphatase that prevents CDKA-1 dephosphorylation and activation. Involved in the hormonal control of cell division and differentiation. Required for proliferation control of meristematic and non-meristematic cells. Negative regulator of the cell cycle.
Gene References into Functions
  1. PEP/PAS2 is an integral membrane protein with cytosolic N- and C-termini. PMID: 20300944
  2. The balance between cell division and differentiation is regulated through the interaction between CDKA;1 and the antiphosphatase PAS2. PMID: 16698944
  3. 3-hydroxy-acyl-CoA dehydratase PASTICCINO2 is an essential and limiting enzyme in VLCFA synthesis but also that PAS2-derived VLCFA homeostasis is required for specific developmental processes PMID: 18799749
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm. Nucleus.
Protein Families
Very long-chain fatty acids dehydratase HACD family
Tissue Specificity
High expression in young seedlings, roots, root tips, flowers and young siliques. Lower levels in leaves and stems.
Database Links

KEGG: ath:AT5G10480

STRING: 3702.AT5G10480.3

UniGene: At.22730

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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