HCH1 Antibody

Code CSB-PA345708XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HCH1 Polyclonal antibody
Uniprot No.
Target Names
HCH1
Alternative Names
HCH1 antibody; YNL281W antibody; N0589 antibody; Hsp90 co-chaperone HCH1 antibody; High-copy Hsp90 suppressor protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HCH1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Co-chaperone that binds to the molecular chaperone HSP82 and stimulates its ATPase activity. Although not essential, it confers thermotolerance when intracellular levels of HSP82 are limiting.
Gene References into Functions
  1. Phosphorylation of human Hsp90alpha at the highly conserved tyrosine (Y) 627 affects it conformation and function. Y627 is not phosphorylated in yeast, but study demonstrated that the non-conserved yeast co-chaperone, Hch1, modifies yeast Hsp90 (Hsp82) through Y606E phosphomimetic mutation raising the possibility that this post-translational modification in higher eukaryotes represents an evolutionary substitution for ... PMID: 28537252
  2. The co-chaperone Hch1 regulates Hsp90 function differently than its homologue Aha1 and confers sensitivity to yeast to the Hsp90 inhibitor NVP-AUY922. PMID: 23166640
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
AHA1 family
Database Links

KEGG: sce:YNL281W

STRING: 4932.YNL281W

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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