HEMC Antibody

Code CSB-PA674861XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HEMC Polyclonal antibody
Uniprot No.
Target Names
HEMC
Alternative Names
HEMC antibody; RUG1 antibody; At5g08280 antibody; F8L15_10 antibody; Porphobilinogen deaminase antibody; chloroplastic antibody; PBG antibody; EC 2.5.1.61 antibody; Hydroxymethylbilane synthase antibody; HMBS antibody; Pre-uroporphyrinogen synthase antibody; Protein RUGOSA1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HEMC protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
Gene References into Functions
  1. These results showed that both porphobilinogen deaminase (HEMC) and multiple organellar RNA editing factor (MORF) proteins are associated with AtECB2 for RNA editing in plastids. PMID: 28850756
  2. The interactions observed between the two pyrrole rings of the HEMC dipyrromethane cofactor and the active-site residues are highly specific and are most likely to represent the catalytically relevant binding mode. PMID: 23519422
  3. Porphobilinogen deaminase deficiency alters vegetative and reproductive development and causes lesions in Arabidopsis. PMID: 23308205
  4. Crystallization and preliminary X-ray characterization of the tetrapyrrole-biosynthetic enzyme porphobilinogen deaminase from Arabidopsis thaliana. [porphobilinogen deaminase] PMID: 23192030
Subcellular Location
Plastid, chloroplast.
Protein Families
HMBS family
Database Links

KEGG: ath:AT5G08280

STRING: 3702.AT5G08280.1

UniGene: At.48996

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