hemG Antibody

Code CSB-PA359742XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) hemG Polyclonal antibody
Uniprot No.
Target Names
hemG
Alternative Names
hemG antibody; yihB antibody; b3850 antibody; JW3827 antibody; Protoporphyrinogen IX dehydrogenase [menaquinone] antibody; EC 1.3.5.3 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) hemG protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX; under anaerobic conditions uses menaquinone as an electron acceptor, under aerobic condition uses ubiquinone as an electron acceptor.; Anaerobically in vitro transfers electrons to fumarate reductase and nitrate reductase; transfer to nitrate reductase couples this reaction to electron transfer across the cell inner membrane and thus ATP synthesis. Neither mesoporphyrinogen nor coproporphyrinogen are substrates. Under aerobic conditions in vitro forms protoporphyrin IX using ubiquinone as an electron acceptor, is able to transfer electrons to cytochrome bd oxidase and cytochrome bo oxidase; transfer to these oxidases couples this reaction to electron transfer across the cell inner membrane and thus ATP synthesis. In cell free extracts deletion of both cytochrome oxidases prevents formation of protoporphyrin IX.
Subcellular Location
Cell inner membrane; Peripheral membrane protein.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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