hgdA Antibody

Code CSB-PA320884XA01AWA
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Product Details

Full Product Name
Rabbit anti-Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4) hgdA Polyclonal antibody
Uniprot No.
Target Names
hgdA
Alternative Names
hgdA antibody; Acfer_1815(R)-2-hydroxyglutaryl-CoA dehydratase antibody; subunit alpha antibody; EC 4.2.1.167; antibody; R)-2-hydroxyglutaryl-CoA dehydratase antibody; component D antibody
Raised in
Rabbit
Species Reactivity
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
Immunogen
Recombinant Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4) hgdA protein
Immunogen Species
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the fermentation of L-glutamate via the hydroxyglutarate pathway. Catalyzes the reversible syn-elimination of water from (R)-2-hydroxyglutaryl-CoA to yield (E)-glutaconyl-CoA. The dehydration mechanism involves a transient one electron reduction of the thioester from (R)-2-hydroxyglutaryl-CoA, generating a ketyl radical. Prior to (E)-glutaconyl-CoA formation, the ketyl radical is subsequently reoxidized by electron transfer back to the HgdA-HgdB complex (CompD) to avoid change in oxidation state of the substrate. The appropriate redox state of dehydratase HgdA-HgdB complex (CompD) is maintained by HgdC (CompA) via hydrolysis of ATP and ATP-dependent electron transfer. Since the electron is recycled, the dehydratase is able to perform several turnovers with only catalytic amounts of ATP and substoichiometric amounts of HgdC (CompA).
Subcellular Location
Cytoplasm.
Protein Families
FldB/FldC dehydratase alpha/beta subunit family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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