hh Antibody

Code CSB-PA461208XA01DLK
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Product Details

Full Product Name
Rabbit anti-Drosophila erecta (Fruit fly) hh Polyclonal antibody
Uniprot No.
Target Names
hh
Alternative Names
hh antibody; GG12458 antibody; Protein hedgehog [Cleaved into: Protein hedgehog N-product; Protein hedgehog C-product] antibody
Raised in
Rabbit
Species Reactivity
Drosophila erecta (Fruit fly)
Immunogen
Recombinant Drosophila erecta (Fruit fly) hh protein
Immunogen Species
Drosophila erecta (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Intercellular signal essential for a variety of patterning events during development. Establishes the anterior-posterior axis of the embryonic segments and patterns the larval imaginal disks. Binds to the patched (ptc) receptor, which functions in association with smoothened (smo), to activate the transcription of target genes wingless (wg), decapentaplegic (dpp) and ptc. In the absence of hh, ptc represses the constitutive signaling activity of smo through fused (fu).; The hedgehog protein N-product constitutes the active species in both local and long-range signaling, whereas the C-terminal product has no signaling activity. It acts as a morphogen, and diffuses long distances despite its lipidation. Heparan sulfate proteoglycans of the extracellular matrix play an essential role in diffusion. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation.; The hedgehog protein C-product, which mediates the autocatalytic activity, has no signaling activity.
Subcellular Location
Nucleus. Cytoplasm.; [Protein hedgehog N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Protein hedgehog C-product]: Secreted, extracellular space.
Protein Families
Hedgehog family
Database Links
CUSABIO guaranteed quality
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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