rasp Antibody

Code CSB-PA893826XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) rasp Polyclonal antibody
Uniprot No.
Target Names
rasp
Alternative Names
rasp antibody; cmn antibody; sit antibody; ski antibody; CG11495 antibody; Protein-cysteine N-palmitoyltransferase Rasp antibody; EC 2.3.1.- antibody; Protein central missing antibody; Protein sightless antibody; Protein skinny hedgehog antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) rasp protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required in hedgehog (hh) expressing cells for production of appropriate signaling activity in embryos and in the imaginal precursors of adult tissues. Acts within the secretory pathway to catalyze N-terminal palmitoylation of Hh; this lipid modification is required for the embryonic and larval patterning activities of the Hh signal. Not required for Wg signaling.
Gene References into Functions
  1. These findings define residues and regions within Shh that are necessary for its recognition as a substrate for Hhat-mediated palmitoylation. PMID: 23112049
  2. Rasp-dependent acylation is necessary to generate a fully active Hh protein. PMID: 11861468
  3. Rasp promotes palmitate addition to the N-terminal cysteine residue of Spitz, and this cysteine is required for Spitz activity in vivo PMID: 16459296
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Membrane-bound acyltransferase family, HHAT subfamily
Database Links

KEGG: dme:Dmel_CG11495

STRING: 7227.FBpp0072893

UniGene: Dm.2681

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