hlh-6 Antibody

Code CSB-PA604538XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans hlh-6 Polyclonal antibody
Uniprot No.
Target Names
hlh-6
Alternative Names
hlh-6 antibody; T15H9.3Helix-loop-helix protein 6 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans hlh-6 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that regulates the development of the g2 pharyngeal gland cells and pharyngeal gland function and thereby is required for feeding. Required for the expression of a number of genes in the pharyngeal gland, possibly by binding to the E box motif (5'-CANNTG-3') in the promoter region of these genes. Positively regulates the expression of genes encoding mucin-like proteins, which lubricate the pharyngeal tract to ensure efficient passage of the bacterial food source. Exhibits pharyngeal gland-specific positive autoregulation activity.
Gene References into Functions
  1. Expression of the gland-expressed genes rely on cis-elements and trans-acting factor(s) other than HLH-6 and HRL3. PMID: 22759833
  2. The experiments identified three distinct regulatory elements required for hlh-6 expression: a PHA-4-binding site and two new elements, HRL1 and HRL2 (for hlh-6 regulatory elements 1 and 2). PMID: 17049341
  3. Pharyngeal pumping in hlh-6 mutants is normal, but hlh-6 mutants lack expression of a class of mucin-related proteins that are normally secreted by pharyngeal glands and line the pharyngeal cuticle. PMID: 18927627
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the gland cells of the pharynx and weakly in the pharyngeal neuron.
Database Links

KEGG: cel:CELE_T15H9.3

STRING: 6239.T15H9.3

UniGene: Cel.14359

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